Expression of tropoelastin in human periodontal ligament fibroblasts after simulation of orthodontic force

Abstract 

The ability of elastic fibers to respond to mechanical stimuli suggests that they play a central role in physiological adaptation to external stimuli including application of orthodontic force. The purpose of this study was to examine the effect of external pressure simulating orthodontic force on tropoelastin gene expression in cultured human periodontal ligament fibroblasts (hPDLF). External pressure simulation was achieved by centrifugation for 10, 30, 60, 90 and 120min of hPDLF in a horizontal microplate rotor. Semi-quantitative RT-PCR analysis of tropoelastin mRNA was performed and β-actin was used as an internal invariant control. While centrifugal force on mRNA levels of β-actin showed almost no change, the mRNA levels of tropoelastin increased significantly to a peak level of more than four-fold after 30min. Thereafter, at 60min, the mRNA levels remained at more than three-fold. After 90min, mRNA levels decreased to control levels. The finding that no changes in mRNA levels of β-actin occurred during the first 90min of centrifugation validates its use as an invariant control gene in such an experimental model. This study demonstrated that tropoelastin is expressed in hPDLF and that the pressure caused significant time-dependent upregulation of the tropoelastin gene. The responsiveness of the tropoelastin gene to force shows its possible clinical importance in orthodontic tooth movement. Further studies, however, are essential in order to learn whether the high expression of the gene in vitro will also be followed by corresponding protein synthesis and deposition in vivo in the extracellular matrix (ECM) of the periodontal ligament (PDL).

Keywords:  Tropoelastin expression, Periodontal ligament cells, Pressure