Family-with-sequence-similarity-46, member A (Fam46a) gene is expressed in developing tooth buds

Etokebe, Godfrey E.; Küchler, Axel M.; Haraldsen, Guttorm; Landin, Maria; Osmundsen, Harald; Dembic, Zlatko

doi:10.1016/j.archoralbio.2009.08.005

« Previous Next »Archives of Oral Biology
Volume 54, Issue 11 , Pages 1002-1007, November 2009

Family-with-sequence-similarity-46, member A (Fam46a) gene is expressed in developing tooth buds

Godfrey E. Etokebe
- Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway
- Corresponding author at: IOB, University of Oslo, PB-1052 Blindern, 0316 Oslo, Norway. Tel.: +47 228 40316; fax: +47 228 40302.
Axel M. Küchler
- LIIPAT, Division of Pathology, Oslo University Hospital, Rikshospitalet, Norway
- Institute of Pathology, University of Oslo, Oslo, Norway
Guttorm Haraldsen
- LIIPAT, Division of Pathology, Oslo University Hospital, Rikshospitalet, Norway
- Institute of Pathology, University of Oslo, Oslo, Norway
Maria Landin
- Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway
Harald Osmundsen
- Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway
Zlatko Dembic
- Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway

Accepted 16 August 2009.

Abstract

Objective

In search for possible novel genes that may be involved in tooth development, we analysed the genome-wide transcriptome of developing mandibular tooth germs of mouse during embryonic and early life and selected family-with-sequence-similarity-46, member A (Fam46a) gene for further expression analysis.

Methods

We applied microarray, quantitative real time polymerase chain reaction and in situ hybridisation methods for the expression study of the mouse Fam46a gene.

Results

We found the family-with-sequence-similarity-46, member A (Fam46a) gene to be highly expressed and further verify its temporo-spatial expression in the mouse tooth.

Conclusion

We have shown that Fam46a is expressed in ameloblasts’ nuclei of tooth germs and hypothesise that it might act together with morphogenetic factors important for the formation of enamel in mouse tooth.

Keywords: Mouse, Fam46a gene, mRNA, Microarray, qPCR, In situ hybridisation