Maxillary sinus floor elevation using a tissue engineered bone complex with BMP-2 gene modified bMSCs and a novel porous ceramic scaffold in rabbits

Sun, X.-Juan; Xia, L.-Guo; Chou, L. Lee; Zhong, Wei; Zhang, X.-Li; Wang, S.-Yi; Zhao, Jun; Jiang, X.-Quan; Zhang, Z.-Yuan

doi:10.1016/j.archoralbio.2010.01.006

« Previous Next »Archives of Oral Biology
Volume 55, Issue 3 , Pages 195-202, March 2010

Maxillary sinus floor elevation using a tissue engineered bone complex with BMP-2 gene modified bMSCs and a novel porous ceramic scaffold in rabbits

X.-Juan Sun
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Department of Oral Maxillofacial Surgery, Affiliated Hospital of Ningxia Medical University, Ningxia 750004, China
- Tel.: +86 954 6743384.
- These authors contributed equally to this work.
L.-Guo Xia
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Tel.: +86 21 23271132.
- These authors contributed equally to this work.
L. Lee Chou
- Division of Oral Medicine, Oral AIDS Clinic Henry M. Goldman School of Dental Medicine, Boston University, 801 Albany Street, S-212, Boston, MA 02118, United States
- L.L. Chou is one of consultants in Yenssen Biotech Company.
- Tel.: +1 617 638 5598.
Wei Zhong
- Shanghai Center for Biomedical Engineering, Shanghai Institutes for Biological Sciences, CAS, Building 14A, 528 Ruiqing Road, Shanghai 201201, China
- Tel.: +86 21 50720318x8056; fax: +86 21 50720318x8002.
X.-Li Zhang
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Tel.: +86 21 23271132.
S.-Yi Wang
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Tel.: +86 21 23271132.
Jun Zhao
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Tel.: +86 21 23271132.
X.-Quan Jiang
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
- Corresponding author at: Shanghai Research Institute of Stomatology, School of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Tel.: +86 21 63135412.
Z.-Yuan Zhang
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China

Accepted 16 January 2010.

Abstract

Objectives

To study the effects of maxillary sinus floor elevation by a tissue engineered bone complex with bone morphogenetic protein-2 (BMP-2) gene modified bone marrow stromal cells (bMSCs) and a novel porous ceramic scaffold (OsteoBone™) in rabbits.

Materials and methods

bMSCs derived from New Zealand rabbit bone marrow were cultured and transduced with adenovirus AdBMP-2 and with AdEGFP gene (without BMP-2 gene sequence) as a control, respectively, in vitro. These bMSCs were then combined with OsteoBone™ scaffold at a concentration of 2×10⁷cells/ml and used to elevate the maxillary sinus floor in rabbits. Eight rabbits were randomly allocated into groups and sacrificed at weeks 2 and 4. For each time point, 8 maxillary sinus floor elevation surgeries were made bilaterally in 4 rabbits for the two groups (n=4 per group): group A (AdBMP-2-bMSCs/material) and group B (AdEGFP-bMSCs/material). All samples were evaluated by histologic and histomorphometric analysis.

Results

The augmented maxillary sinus height was maintained for both groups over the entire experimental period, while new bone area increased over time for group A. At week 4 after operation, bone area in group A was significantly more than that in group B (P<0.05), and was more obviously detected in the center of the elevated space. Under a confocal microscope, green fluorescence in newly formed bone was observed in the EGFP group, which suggests that those implanted bMSCs had contributed to the new bone formation.

Conclusion

bMSCs modified with AdBMP-2 gene can promote new bone formation in elevating the rabbit maxillary sinus. OsteoBone™ scaffold could be an ideal carrier for gene enhanced bone tissue engineering.

Keywords: Bone marrow stromal cells, Bone morphogenetic protein, Gene therapy, Sinus floor elevation, Tissue engineering scaffold