Archives of Oral Biology
Volume 55, Issue 4 , Pages 268-278 , April 2010

Identification of salivary proteins at oil–water interfaces stabilized by lysozyme and β-lactoglobulin

  • Erika Silletti

      Affiliations

    • Top Institute Food and Nutrition PO Box 557, 6700 AN Wageningen, The Netherlands
    • NIZO Food Research BV, Postbus 20, 6710 BA Ede, The Netherlands
    • Corresponding Author InformationCorresponding author at: Top Institute Food and Nutrition PO Box 557, 6700 AN Wageningen, The Netherlands. Tel.: +31 317 482101; fax: +31 317 484893.
    • ,
  • Rui M.P. Vitorino

      Affiliations

    • Department of Chemistry, University of Aveiro, ZIP-3810-193, Aveiro, Portugal
    • ,
  • Raymond Schipper

      Affiliations

    • Top Institute Food and Nutrition PO Box 557, 6700 AN Wageningen, The Netherlands
    • Laboratory of Food Chem, Wageningen University and Research Centre, PO Box 8129, 6700 EV Wageningen, The Netherlands
    • ,
  • Francisco M.L. Amado

      Affiliations

    • Department of Chemistry, University of Aveiro, ZIP-3810-193, Aveiro, Portugal
    • ,
  • Monique H. Vingerhoeds

      Affiliations

    • Top Institute Food and Nutrition PO Box 557, 6700 AN Wageningen, The Netherlands
    • Wageningen UR Food and Biobased Research, PO Box 17, 6700 AA Wageningen, The Netherlands

,Accepted 4 February 2010.

References 

  1. Engelen L, de Wijk RA, Prinz JF, Bosman F. The relation between saliva flow after different stimulations and the perception of flavor and texture attributes in custard desserts. Physiol Behav. 2003;78:165–169
  2. Engelen L, de Wijk RA, Prinz JF, Janssen AM, van der Bilt A, Weenen H, et al. A comparison of the effects of added saliva, alpha-amylase and water on texture perception in semisolids. Physiol Behav. 2003;78:805–811
  3. Kallithraka S, Bakker J, Clifford MN, Vallis L. Correlations between saliva protein composition and some T–I parameters of astringency. Food Qual Prefer. 2001;12:145–152
  4. Guinard J-X, Zoumas-Morse C, Walchak C. Relation between parotid saliva flow and composition and the perception of gustatory and trigeminal stimuli in foods. Physiol Behav. 1998;63:109–118
  5. Charlton AJ, Baxter NJ, Lilley TH, Haslam E, McDonald CJ, Williamson MP. Tannin interactions with a full-length human salivary proline-rich protein display a stronger affinity than with single proline-rich repeats. FEBS Lett. 1996;382:289–292
  6. Lu Y, Bennick A. Interaction of tannin with human salivary proline-rich proteins. Arch Oral Biol. 1998;43:717–728
  7. de Freitas V, Mateus N. Structural features of procyanidin interactions with salivary proteins. J Agric Food Chem. 2001;49:940–945
  8. Gambuti A, Rinaldi A, Pessina R, Moio L. Evaluation of aglianico grape skin and seed polyphenol astringency by SDS-PAGE electrophoresis of salivary proteins after the binding reaction. Food Chem. 2006;97:614–620
  9. Silletti E, Vingerhoeds MH, Norde W, van Aken GA. The role of electrostatics in saliva-induced emulsion flocculation. Food Hydrocolloid. 2007;21:596–606
  10. Silletti E, Vingerhoeds MH, van Aken GA, Norde W. Rheological behaviour of food emulsions mixed with saliva: effect of oil content, salivary protein content and saliva type. Food Biophys. 2008;3:318–328
  11. Vingerhoeds MH, Silletti E, de Groot J, Schipper R, van Aken GA. Relating the effect of saliva-induced emulsion flocculation on rheological properties and retention on the tongue surface with sensory perception. Food Hydrocolloid. 2009;23:773–785
  12. Mela DJ, Langley KR, Martin A. Sensory assessment of fat content: effect of emulsion and subject characteristics. Appetite. 1994;22:67–81
  13. Silletti E, Vingerhoeds MH, Norde W, van Aken GA. Complex formation in mixtures of lysozyme-stabilized emulsions and human saliva. J Colloid Interface Sci. 2007;313:485–493
  14. Hu S, Loo JA, Wong DT. Human saliva proteome analysis. 2007;p. 323–9
  15. Vitorino R, Barros A, Caseiro A, Domingues P, Duarte J, Amado F. Towards defining the whole salivary peptidome. Proteomics Clin Appl. 2009;3:528–540
  16. De Smet K, Contreras R. Human antimicrobial peptides: defensins, cathelicidins and histatins. Biotechnol Lett. 2005;27:1337–1347
  17. Humphrey SP, Williamson RT. A review of saliva: normal composition, flow and function. J Prosthet Dent. 2001;85:162–169
  18. van Nieuw Amerongen A, Bolscher JGM, Veerman ECI. Salivary proteins: protective and diagnostic value in cardiology?. Caries Res. 2004;38:247–253
  19. Raynal BDE, Hardingham TE, Thornton DJ, Sheehan JK. Concentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva. Biochem J. 2002;362:289–296
  20. Helmerhorst EJ, Oppenheim FG. Saliva: a dynamic proteome. J Dent Res. 2007;86:680–693
  21. Levine MJ, Reddy MS, Tabak LA, Loomis RE, Bergey EJ, Jones PC, et al. Structural aspects of salivary glycoproteins. J Dent Res. 1987;66:436–441
  22. Loomis RE, Prakobphol A, Levine MJ, Jones PC. Biochemical and biophysical comparison of two mucins from human submandibular-sublingual saliva. Arch Biochem Biophys. 1987;258:452–464
  23. Zalewska A, Zwierz K, Zólkowski K, Gindzienski A. Structure and biosynthesis of human salivary mucins. Acta Biochim Pol. 2000;47:1067–1079
  24. Bobek LA, Tsai H, Biesbrock AR, Levine MJ. Molecular cloning, sequence and specificity of expression of the gene encoding the low molecular weight salivary mucin (MUC7). J Biol Chem. 1993;268:20563–20569
  25. Reddy MS, Bobek LA, Haraszthy GG, Biesbrock AR, Levine MJ. Structural features of the low-molecular-mass human salivary mucin. Biochem J. 1992;287:639–643
  26. Reddy MS, Levine MJ, Prakobphol A. Oligosaccharide structures of the low-molecular-weight salivary mucin from a normal individual and one with cystic fibrosis. J Dent Res. 1985;64:33–36
  27. Thornton DJ, Khan N, Mehrotra R, Howard M, Veerman ECI, Packer NH, et al. Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Glycobiology. 1999;9:293–302
  28. Thomsson KA, Prakobphol A, Leffler H, Reddy MS, Levine MJ, Fisher SJ, et al. The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Glycobiology. 2002;12:1–14
  29. Veerman ECI, van den Keijbus PAM, Nazmi K, Vos W, van der Wal JE, Bloemena E, et al. Distinct localization of MUC5B glycoforms in the human salivary glands. Glycobiology. 2003;13:363–366
  30. Veerman ECI, van den Keybus PAM, Valentijn-Benz M, van Nieuw Amerongen A. Isolation of different high-Mr mucin species from human whole saliva. Biochem J. 1992;283:807–811
  31. Wickström C, Davies JR, Eriksen GV, Veerman ECI, Carlstedt I. MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: identification of glycoforms and C-terminal cleavage. Biochem J. 1998;334:685–693
  32. Schipper RG, Silletti E, Vingerhoeds MH. Saliva as research material: biochemical, physicochemical and practical aspects. Arch Oral Biol. 2007;52:1114–1135
  33. Banderas-Tarabay JA, Zacarias-D’Oleire IG, Garduno-Estrada R, Aceves-Luna E, Gonzalez-Begne M. Electrophoretic analysis of whole saliva and prevalence of dental caries. A study in Mexican dental students. Arch Med Res. 2002;33:499–505
  34. Beeley JA, Sweeney D, Lindsay JCB, Buchanan ML, Sarna L, Khoo KS. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins. Electrophoresis. 1991;12:1032–1041
  35. Minaguchi K, Madapallimattam G, Bennick A. The presence and origin of phosphopeptides in human saliva. Biochem J. 1988;250:171–177
  36. Veerman ECI, van den Keybus PAM, Vissink A, vanNieuw Amerongen A. Human glandular salivas: their separate collection and analysis. Eur J Oral Sci. 1996;104:346–352
  37. Iontcheva I, Oppenheim FG, Troxler RF. Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res. 1997;76:734–743
  38. Soares RV, Lin T, Siqueira CC, Bruno LS, Li X, Oppenheim FG, et al. Salivary micelles: identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycolsylated proline-rich protein and lysozyme. Arch Oral Biol. 2004;49:337–343
  39. Jensen JL, Lamkin MS, Oppenheim FG. Adsorption of human salivary proteins to hydroxyapatite: a comparison between whole saliva and glandular salivary secretions. J Dent Res. 1992;71:1569–1576
  40. Schwartz SS, Zhu WX, Sreebny LM. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human whole saliva. Arch Oral Biol. 1995;40:9499–9558
  41. Proctor GB, Hamdan S, Carpenter GH, Wilde PJ. A statherin and calcium enriched layer at the air interface of human parotid saliva. Biochem J. 2005;389:111–116
  42. Bolscher JGM, Groenink J, van der Kwaak JS, van den Keijbus PAM, van’t Hof W, Veerman ECI, et al. Detection and quantification of MUC7 in submandibular, sublingual, palatine and labial saliva by anti-peptide antiserum. J Dent Res. 1999;78:1362–1369
  43. Veerman EC, Bolscher JG, Appelmelk BJ, Bloemena E, van den Berg TK, van Nieuw Amerongen A. A monoclonal antibody directed against high M(r) salivary mucins recognizes the SO3-3Gal beta 1-3GlcNAc moiety of sulfo-Lewis(a): a histochemical survey of human and rat tissue. Glycobiology. 1997;7:37–43
  44. Skingsley DR. Fourier-transform infrared (FTIR) spectroscopic methods for low volume analysis of oral mucus. Eur Respir J. 2003;22:446s
  45. Skingsley DR, Sheffield D. Investigation of human oral mucus using Fourier-transform infrared (FTIR) spectroscopy. Eur Respir J. 2002;20:175s
  46. de Jongh HHJ, Janssen AM. Differential clearance kinetics of adhered layer constituents from the oral cavity as modulator for after feel of dressings: ATR FT-IR measurements of localized oral coatings. J Texture Stud. 2007;38:70–86
  47. Silletti E. When emulsions meet saliva. A physical–chemical, biochemical and sensory study [doctoral dissertation]. Wageningen: Wageningen University; 2008.
  48. de Jongh HHJ, Groneveld T, de Groot J. Mild isolation procedure discloses new protein structural properties of beta-lactoglobulin. J Dairy Sci. 2001;84:562–571
  49. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985;150:76–85
  50. Schipper R, Loof A, de Groot J, Harthoorn L, Dransfield E, van Heerde W. SELDI-TOF-MS of saliva: methodology and pre-treatment effects. J Chromatogr B. 2007;847:45–53
  51. Kapitany RA, Zebrowski EJ. A high resolution PAS stain for polyacrylamide gel electrophoresis. Anal Biochem. 1973;56:361–369
  52. Vitorino R, de Morais Guedes S, Ferreira R, Lobo MJC, Duarte J, Ferrer-Correia AJ, et al. Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility. Eur J Oral Sci. 2006;114:147–153
  53. Vitorino R, Calheiros-Lobo MJ, Williams J, Ferrer-Correia AJ, Tomer KB, Duarte J, et al. Peptidomic analysis of human acquired enamel pellicle. Biomed Chromatogr. 2007;21:1107–1117
  54. Vitorino R, Lobo MJC, Duarte J, Domingues PM, Amado FML. Peptide profile of human acquired enamel pellicle using MALDI tandem MS. J Sep Sci. 2008;3:523–537
  55. Hardt M, Thomas LR, Newport G, Agabian N, Prakobphol A, Hall SC, et al. Toward defining the human parotid gland salivary proteome and peptidome: identification and characterization using 2D SDS-PAGE, ultrafiltration, HPLC, and mass spectrometry. Biochemistry. 2005;44:2885–2899
  56. Vitorino R, Lobo MJC, Ferre-Correira AJ, Dubin JR, Tomer KB, Domingues PM, et al. Identification of human whole saliva protein components using proteomics. Proteomics. 2004;4:1109–1115
  57. Amado FML, Vitorino RMP, Domingues PMDN, Lobo MJC, Duarte JAR. Analysis of the human saliva proteome. Expert Rev Proteomics. 2005;2:521–539
  58. Dedinaite A, Lundin M, Macakova L, Auletta T. Mucin–chitosan complexes at the solid–liquid interface: multilayer formation and stability in surfactant solutions. Langmuir. 2005;21:9502–9509
  59. Lu JR, Su TJ, Thomas RK, Penfold J, Webster J. Structural conformation of lysozyme layers at the air/water interface studied by neutron reflection. J Chem Soc Faraday Trans. 1998;94:3279–3287
  60. De Roos AL, Walstra P. Loss of enzyme activity due to adsorption onto emulsion droplets. Colloids Surf B: Biointerfaces. 1996;6:201–208
  61. Schmitt C, Sanchez C, Desobry-Banon S, Hardy J. Structure and technofunctional properties of protein–polysaccharide complexes: a review. Crit Rev Food Sci Nutr. 1998;38:689–753
  62. Lendenmann U, Grogan J, Oppenheim FG. Saliva and dental pellicle—a review. Adv Dent Res. 2000;14:22–28
  63. Lamkin MS, Migliari D, Yao Y, Troxler RF, Oppenheim FG. New in vitro model for the acquired enamel pellicle: pellicles formed from whole saliva show inter-subject consistency in protein composition and proteolytic fragmentation patterns. J Dent Res. 2001;80:385–388
  64. Yao Y, Berg EA, Costello CE, Troxler RF, Oppenheim FG. Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches. J Biol Chem. 2003;278:5300–5308
  65. Yao Y, Grogan J, Zehnder M, Lendenmann U, Nam B, Wu Z, et al. Compositional analysis of human acquired enamel pellicle by mass spectrometry. Arch Oral Biol. 2001;46:293–303
  66. Siqueira WL, Helmerhorst EJ, Zhang W, Salih E, Oppenheim FG. Acquired enamel pellicle and its potential role in oral diagnostics. Ann N Y Acad Sci. 2007;1098:504–509
  67. Lamkin MS, Arancillo AA, Oppenheim FG. Temporal and compositional characteristics of salivary protein adsorption to hydroxyapatite. J Dent Res. 1996;75:803–808
  68. Embery G, Heaney TG, Stanbury JB. Studies on the organic polyanionic constituents of human acquired dental pellicle. Arch Oral Biol. 1986;31:623–625
  69. Carvalho E, Mateus N, Plet B, Pianet I, Dufourc E, de Freitas V. Influence of wine pectic polysaccharides on the interactions between condensed tannins and salivary proteins. J Agric Food Chem. 2006;54:8936–8944

PII: S0003-9969(10)00031-2

doi: 10.1016/j.archoralbio.2010.02.004

Archives of Oral Biology
Volume 55, Issue 4 , Pages 268-278 , April 2010

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