Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle

Hannig, C.; Spitzmüller, B.; Lux, H.C.; Altenburger, M.; Al-Ahmad, A.; Hannig, M.

doi:10.1016/j.archoralbio.2010.03.020

« Previous Next »Archives of Oral Biology
Volume 55, Issue 7 , Pages 463-469, July 2010

Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle

C. Hannig
- Clinic of Conservative Dentistry, Faculty of Medicine ‘Carl Gustav Carus’, Technical University of Dresden, 01307 Dresden, Germany
- Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany
- Corresponding author at: Clinic of Conservative Dentistry, University Hospital, University of Dresden, Fetscherstr. 74, 01307 Dresden, Germany. Tel.: +49 351 458 2713; fax: +49 351 458 5318.
B. Spitzmüller
- Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany
H.C. Lux
- Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany
M. Altenburger
- Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany
A. Al-Ahmad
- Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany
M. Hannig
- Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, Germany

Accepted 24 March 2010.

Abstract

Aim

Different enzyme-containing toothpastes are available on the market. The aim of the present in situ study was to investigate their efficacy for immobilisation of protective enzymes in the pellicle layer.

Methods

Pellicle formation took place in situ on bovine enamel slabs fixed to individual upper jaw splints carried by 6 subjects. After pellicle formation for 1min, brushing was performed for 3min with the commercially available toothpastes Enzycal, biotène and BioXtra, respectively. Before as well as 0, 20 and 40min after brushing, samples were removed from the splints and tested for lysozyme, peroxidase and glucoseoxidase activity. The assays for the respective enzyme activities were based on fluorogenic substrates. Separate experiments were conducted for the different enzymes and toothpastes.

Results

Brushing with the toothpastes caused an extensive increase of glucoseoxidase activity in the pellicle, but it was of low tenacity whereas peroxidase activity was enhanced considerably. However, targeted accumulation of lysozyme in the pellicle was not very pronounced. Brushing without toothpaste had no effect on enzyme activities in the acquired pellicle.