Gingival overgrowth in cyclosporine, tacrolimus, or sirolimus-based immunosuppressive regimens and the single nucleotide IL-6 (−174 G/C) gene polymorphism

Cota, Luís Otávio Miranda; Viana, Michelle Beatriz; Moreira, Paula Rocha; Gomez, Ricardo Santiago; Cortelli, José Roberto; Cortelli, Sheila Cavalca; Costa, Fernando Oliveira

doi:10.1016/j.archoralbio.2010.04.001

« Previous Next »Archives of Oral Biology
Volume 55, Issue 7 , Pages 494-501, July 2010

Gingival overgrowth in cyclosporine, tacrolimus, or sirolimus-based immunosuppressive regimens and the single nucleotide IL-6 (−174 G/C) gene polymorphism

Luís Otávio Miranda Cota
- Department of Dental Clinics, Oral Pathology and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
- Avenida Antonio Carlos, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil
Michelle Beatriz Viana
- Department of Dental Clinics, Oral Pathology and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
- Avenida Antonio Carlos, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil
Paula Rocha Moreira
- Department of Dental Clinics, Oral Pathology and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
- Avenida Antonio Carlos, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil
Ricardo Santiago Gomez
- Department of Dental Clinics, Oral Pathology and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
- Avenida Antonio Carlos, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil
José Roberto Cortelli
- Department of Dentistry, Periodontics Research Division, University of Taubaté, Taubaté, São Paulo, Brazil
- Avenida Nove de Julho, 245 Centro, Zip Code 12020-200, Taubaté, SP, Brazil
Sheila Cavalca Cortelli
- Department of Dentistry, Periodontics Research Division, University of Taubaté, Taubaté, São Paulo, Brazil
- Avenida Nove de Julho, 245 Centro, Zip Code 12020-200, Taubaté, SP, Brazil
Fernando Oliveira Costa
- Department of Dental Clinics, Oral Pathology and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
- Avenida Antonio Carlos, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil
- Corresponding author at: Federal University of Minas Gerais, Department of Dental Clinics, Oral Pathology and Oral Surgery, Antonio Carlos Avenue, 6627 Pampulha, PO Box 359, Zip Code 31270-901, Belo Horizonte, MG, Brazil. Tel.: +55 31 3409 2412x2427; fax: +55 31 3282 6787.

Accepted 6 April 2010.

Abstract

Objective

Interleukin-6 (IL-6) may be involved in drug-induced gingival overgrowth (GO). The present study was conducted to assess the association between IL-6 (−174 G/C) gene polymorphism and GO in renal transplant recipients under cyclosporine (CsA), tacrolimus (Tcr), or sirolimus (Sir)-based regimens.

Methods

Within an eligible population, 45 unrelated subjects were selected for each CsA, Tcr, and Sir group, totaling a sample of 135 subjects. GO was visually assessed and subjects were assigned as controls (non-responders) or cases (responders) in a post hoc definition. IL-6 gene polymorphism was assessed using the polymerase chain reaction amplification and digestion. The distribution of genotypes and allele frequencies in responders and non-responders were compared using the Chi-squared test.

Results

The number of responders was 27 (60.0%), 13 (28.9%), and 7 (15.6%) in the CsA, Tcr, and Sir groups, respectively. No differences could be observed at frequencies of −174GG, −174CG, and −174CC genotypes when comparing responders to non-responders in the CsA, Tcr, and Sir groups. Similar to genotypes, allele frequencies showed no differences between responders and non-responders in all groups.

Conclusions

No association between IL-6 (−174 G/C) gene polymorphism and gingival overgrowth was observed in renal transplant recipients under CsA, Tcr, or Sir-based immunosuppressive maintenance regimens.

Keywords: Immunosuppressants, Gingival overgrowth, Interleukin-6, Polymorphism, Renal transplantation