Astaxanthin restores the enzymatic antioxidant profile in salivary gland of alloxan-induced diabetic rats

Leite, Mariana Ferreira; Lima, Amanda Martins de; Massuyama, Monica Miyuki; Otton, Rosemari

doi:10.1016/j.archoralbio.2010.04.006

« Previous Next »Archives of Oral Biology
Volume 55, Issue 7 , Pages 479-485, July 2010

Astaxanthin restores the enzymatic antioxidant profile in salivary gland of alloxan-induced diabetic rats

Mariana Ferreira Leite
- Pediatric Dentistry Ph.D. Program, Ciências Biológicas e da Saúde, Universidade Cruzeiro do Sul, Avenida Ussiel Cirilo, 225, CEP 08060-070, São Paulo, Brazil
- Corresponding author at: Ciências Biológicas e da Saúde – CBS Universidade Cruzeiro do Sul. Rua Aurélia, 1821, apto121 – Vila Romana, São Paulo, SP, CEP 05046-001, Brazil. Tel.: +55 11 78727095.
Amanda Martins de Lima
- Pediatric Dentistry Ph.D. Program, Ciências Biológicas e da Saúde, Universidade Cruzeiro do Sul, Avenida Ussiel Cirilo, 225, CEP 08060-070, São Paulo, Brazil
Monica Miyuki Massuyama
- Pediatric Dentistry Ph.D. Program, Ciências Biológicas e da Saúde, Universidade Cruzeiro do Sul, Avenida Ussiel Cirilo, 225, CEP 08060-070, São Paulo, Brazil
Rosemari Otton
- Postgraduate Program – Health Science, CBS, Universidade Cruzeiro do Sul, São Paulo, SP 03342-000, Brazil

Accepted 29 April 2010.

Abstract

Objective

To evaluate the effect of astaxanthin on antioxidant parameters of salivary gland from diabetic rats. The hypothesis of the study was whether the supplementation of diabetic rats with astaxanthin might antagonize, or at least prevent, the defect in their antioxidative status.

Design

Wistar rats (n=32) were divided in 4 groups: untreated control, treated control, untreated diabetic and treated diabetic rats. Astaxanthin (20mg/kg body weight) was administered daily by gavage for 30 days. On day 23, diabetes was induced by injection of alloxan (60mg/kg body weight). After 7 days of diabetes induction, the rats were killed and submandibular and parotid removed. Superoxide dismutase (SOD), catalase, glutathione peroxidase and reductase activities and the content of thiol groups were determined. Data were compared by ANOVA and the Tukey test (p<0.05).

Results

Diabetes caused a reduction of SOD, and thiol content and increase of catalase and glutathione peroxidase activities of submandibular gland whilst in the parotid gland diabetes caused an increase of thiol content and no effect in the antioxidant system. The astaxanthin restores the enzymatic activities in the salivary gland, however does not prevent its oxidative damage.

Conclusion

The submandibular gland presented more susceptibility to oxidative alterations induced by diabetes. Astaxanthin presented a positive effect on the oxidative protection of the salivary gland from diabetic rats.

Keywords: Salivary gland, Antioxidant system, Diabetes, Astaxanthin