The role of Desulfovibrio desulfuricans lipopolysaccharides in modulation of periodontal inflammation through stimulation of human gingival fibroblasts

Dzierżewicz, Zofia; Szczerba, Joanna; Lodowska, Jolanta; Wolny, Daniel; Gruchlik, Arkadiusz; Orchel, Arkadiusz; Węglarz, Ludmiła

doi:10.1016/j.archoralbio.2010.05.001

« Previous Next »Archives of Oral Biology
Volume 55, Issue 7 , Pages 515-522, July 2010

The role of Desulfovibrio desulfuricans lipopolysaccharides in modulation of periodontal inflammation through stimulation of human gingival fibroblasts

Zofia Dzierżewicz
- Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
Joanna Szczerba
- Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
Jolanta Lodowska
- Department of Biochemistry, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
Daniel Wolny
- Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
- Corresponding author. Tel.: +48 323641060; fax: +48 323641060.
Arkadiusz Gruchlik
- Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
Arkadiusz Orchel
- Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland
Ludmiła Węglarz
- Department of Biochemistry, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland

Accepted 6 May 2010.

Abstract

Objective

Periodontitis is a destructive disease which is likely to be the result of the activities of different microbial complexes. Recently, sulphate-reducing bacteria (SRB) have been detected in the oral cavity, and they have been found to be common inhabitants of sites showing periodontal destruction. The aim of study was to evaluate the influence of endotoxins of Desulfovibrio desulfuricans bacteria on human gingival fibroblast HGF-1 line.

Methods

The immunological response of gingival fibroblasts was evaluated by determination of their IL-6 and IL-8 secretion upon treatement with D. desulfuricans intestinal and type strain LPS, sodium butyrate (NaB) and IL-1β. The amounts of cytokines were estimated by ELISA immunoassay. The influence of LPS and NaB on fibroblast proliferation was determined using the CyQUANT Cell Proliferation Assay Kit.

Results

No significant growth inhibition of cells exposed to LPS was observed, except for the culture growing in the presence of intestinal strain endotoxin at the highest concentration (100μg/ml). The secretion of IL-6 and IL-8 by fibroblasts was increased by D. desulfuricans endotoxins. Cells stimulated with proinflammatory cytokine 1L-1β showed very high levels of both cytokines secretion. The release of IL-6 and IL-8 by cells in response to LPS and 1L-1β was modulated by butyric acid.

Conclusions

The observed response of gingival fibroblasts to stimulation by endotoxin suggests that D. desulfuricans can be involved in the pathogenesis of periodontitis. Moreover, butyrate present in the oral cavity seems to have immunoregulatory effect on cytokine production by gingival fibroblasts under physiological conditions and during microbe-induced inflammation.

Keywords: Cytokine, Desulfovibrio desulfuricans, HGF-1 line, Lipopolysaccharide, Periodontitis