Archives of Oral Biology
Volume 55, Issue 8 , Pages 570-575 , August 2010

Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples

  • E. Matalova

      Affiliations

    • Department of Physiology and Pathophysiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
    • Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic
    • Corresponding Author InformationCorresponding author at: Laboratory of Animal Embryology, Institute of Animal Physiology and Genetics CAS, v.v.i., Veveri 97, 60200 Brno, Czech Republic. Tel.: +420 532290155.
    • ,
  • L. Dubska

      Affiliations

    • Masaryk Memorial Cancer Institute, Brno, Czech Republic
    • ,
  • J. Fleischmannova

      Affiliations

    • Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic
    • ,
  • I. Chlastakova

      Affiliations

    • Department of Physiology and Pathophysiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
    • Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic
    • ,
  • E. Janeckova

      Affiliations

    • Department of Physiology and Pathophysiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
    • Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic
    • ,
  • A.S. Tucker

      Affiliations

    • Department of Craniofacial Development and Orthodontics, KCL, London, UK

,Accepted 17 May 2010.

References 

  1. Coucouvanis E, Martin GR. Signals for death and survival: a two-step mechanism for cavitation in the vertebrate embryo. Cell. 1995;83:279–287
  2. Doseff AI. Apoptosis: the sculptor of development. Stem Cells Dev. 2004;13:473–483
  3. Stiles AD, Chrysis D, Jarvis HW, Brighton B, Moats-Staats BM. Programmed cell death in normal fetal rat lung development. Exp Lung Res. 2001;27:569–587
  4. Hayashi M, Araki T. Caspase in renal development. Nephrol Dial Transplant. 2002;17:8–10
  5. Melnick M, Jaskoll T. Mouse submandibular gland morphogenesis: a paradigm for embryonic signal processing. Crit Rev Oral Biol Med. 2000;11:199–215
  6. Fulton N, Martins da Silva SJ, Bayne RA, Anderson RA. Germ cell proliferation and apoptosis in the developing human ovary. J Clin Endocrinol Metab. 2005;90:4664–4670
  7. Boonman Z, Isacson O. Apoptosis in neuronal development and transplantation: role of caspases and trophic factors. Exp Neurol. 1999;156:1–15
  8. Poelmann RE, Gittenberger-de Groot AC. Apoptosis as an instrument in cardiovascular development. Birth Defects Res C: Embryo Today. 2005;75:305–313
  9. Siegel RM. Caspases at the crossroads of immune-cell life and death. Nat Rev Immunol. 2006;6:308–317
  10. Matalova E, Tucker AS, Sharpe PT. Death in the life of a tooth. J Dent Res. 2004;83:11–16
  11. Peterkova R, Peterka M, Lesot H. The developing mouse dentition: a new tool for apoptosis study. Ann N Y Acad Sci. 2003;1010:453–466
  12. Jernvall J, Kettunen P, Karavanova I, Martin LB, Thesleff I. Evidence for the role of the enamel knot as a control center in mammalian tooth cusp formation – non-dividing cells express growth-stimulating Fgf-4 gene. Int J Dev Biol. 1994;38:463–469
  13. Thesleff I, Keranen S, Jernvall J. Enamel knot as signalling centres linking tooth morphogenesis and odontoblast differentiation. Adv Dent Res. 2001;15:14–18
  14. Lesot H, Vonesch JL, Peterka M, Tureckova J, Peterkova R, Ruch JV. Mouse molar morphogenesis revised by three-dimensional reconstruction. II. Spatial distribution of mitoses and apoptosis in cap to bell stages first and second upper molar teeth. Int J Dev Biol. 1996;40:1017–1031
  15. Setkova J, Lesot H, Matalova E, Witter K, Matulova P, Misek I. Proliferation and apoptosis in early molar morphogenesis – voles as models in odontogenesis. Int J Dev Biol. 2006;50:481–489
  16. Jernvall J, Aberg T, Kettunen P, Keranen S, Thesleff I. The life history of an embryonic signaling center: BMP-4 induces p21 and is associated with apoptosis in the mouse tooth enamel knot. Development. 1998;125:161–169
  17. Kieffer S, Peterkova R, Vonesh JH, Ruch JV, Peterka M, Lesot H. Morphogenesis of the lower incisor in the mouse from the bud to early bell stage. Int J Dev Biol. 1999;43:531–539
  18. Shigemura N, Kiyoshima T, Sakai T, Matsuo K, Momoi T, Yamaza H, et al. Localization of activated caspase-3-positive and apoptotic cells in the developing tooth germ of the mouse lower first molar. Histochem J. 2001;33:253–258
  19. Sasaki C, Sato T, Kozawa Y. Apoptosis in regressive deciduous tooth germs of Suncus murinus evaluated by the TUNEL method and electron microscopy. Arch Oral Biol. 2001;46:649–660
  20. Shigemura N, Kiyoshima T, Kobayashi I, Matsuo K, Yamaza H, Akamine A, et al. The distribution of BrdU- and TUNEL-positive cells during odontogenesis in mouse lower first molars. Biochem J. 1999;31:367–377
  21. Matalova E, Kovaru F, Misek I. Caspase 3 activation in the primary enamel knot of developing molar tooth. Physiol Res. 2006;55:183–188
  22. Matalova E, Sharpe PT, Lakhani SA, Roth KA, Flavell RA, Setkova J, et al. Molar tooth development in caspase-3 deficient mice. Int J Dev Biol. 2006;50:491–497
  23. Simone NL, Bonner RF, Gillespie JW, Emmert-Buck MR, Liotta LA. Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet. 1998;14:272–276
  24. Fend F, Raffeld M. Laser capture microdissection in pathology. J Clin Pathol. 2000;53:666–672
  25. Domazet B, Maclennan GT, Lopez-Beltran A, Montironi R, Cheng L. Laser-capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer. Int J Clin Exp Pathol. 2008;1:475–488
  26. Craven RA, Totty N, Hamden P, Selby PJ, Banks RE. Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis – evaluation of tissue preparation and sample limitations. Am J Pathol. 2002;160:815–822
  27. Lee JR, Baxter TM, Yamagushi H, Wang TC, Goldenring JR, Anderson MG. Differential protein analysis of spasomolytic polypeptide expressing metaplasma using laser capture microdissection and two-dimensional difference gel electrophoresis. Appl Immunohistochem Mol Morphol. 2003;11:188–193
  28. Bhattacharya SH, Gal AA, Murray KK. Laser capture microdissection MALDI for direkt analysis of archival tissue. J Proteom Res. 2003;2:95–98
  29. Espina V, Dettloff KA, Cowherd S, Petricoin EF, Lotta LA. Use of proteomic analysis to monitor responses to biological therapies. Exp Opin Biol Ther. 2004;4:83–93
  30. Spisak S, Guttman A. Biomedical applications of protein microarrays. Cur Med Chem. 2009;16:2806–2815
  31. Craven RA, Banks RE. Laser capture microdissection and proteomics: possibilities and limitation. Proteomics. 2001;1:1200–1204
  32. Murray GI. An overview of laser microdissection technologies. Acta Histochem. 2007;109:171–176
  33. Banks RE, Dunn MJ, Forbes MA, Stanley A, Pappin D, Naven T, et al. The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis – preliminary findings. Electrophoresis. 1999;20:689–700
  34. DiFrancesco LM, Murthy SK, Luider J, Demetrick DJ. Laser capture microdissection-guided fluorescence in situ hybridization and flow cytometric cell cycle analysis of purified nuclei from paraffin sections. Modern Pathol. 2000;13:705–711
  35. Bellet V, Boissiere F, Bibeau F, Desmetz C, Berthe ML, Rochaix P, et al. Proteomic analysis of RCL paraffin-embedded tissues. J Cell Mol Med. 2008;12:2027–2036
  36. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang ZP, Goldstein SR, et al. Laser capture microdissection. Science. 1996;274:998–1001
  37. Ornstein DK, Gillespie JW, Paweletz CP, Duray PH, Herring J, Vocke CD, et al. Proteomic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines. Electrophoresis. 2000;21:2235–2242
  38. Matalova E, Spanova A. Detection of apoptotic DNA ladder in pig leukocytes and its precision using LM-PCR (ligation mediated polymerase chain reaction). Acta Vet Brno. 2002;71:163–168
  39. Leary JF, Szaniszlo P, Prow T, Reece LM, Wang N, Asmuth DM. The importance of high-throughput cell separation technologies for genomics/proteomics-based clinical diagnostics. Clin Diag Syst. 2002;4625:1–8
  40. Valet G, Leary JF, Tarnok A. Cytomics – new technologies: toward a human cytome project. Cytometry. 2004;59A:167–171
  41. Herrera G, Diaz L, Martinez-Romero A, Gomes A, Villamon E, Callaghan RC, et al. Cytomics: a multiparametric, dynamic approach to cell research. Tox in vitro. 2007;21:176–182

PII: S0003-9969(10)00129-9

doi: 10.1016/j.archoralbio.2010.05.007

Archives of Oral Biology
Volume 55, Issue 8 , Pages 570-575 , August 2010

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